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DNA quantification

DRAFT

This page is a work in progress and is subject to change at any moment.

Accurate quantification and quality assessment of the extracted DNA are crucial:

  • Spectrophotometric methods: UV absorbance at 260 nm is used to quantify DNA, with the 260/280 nm ratio indicating purity.
  • Fluorometric assays: Dye-based methods (e.g., PicoGreen) offer higher sensitivity and specificity for double-stranded DNA.
  • Gel electrophoresis: Provides information on DNA integrity and can detect contamination by RNA or degraded DNA.