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Adapter ligation

DRAFT

This page is a work in progress and is subject to change at any moment.

These steps prepare the DNA fragments for sequencing:

  • End repair: Converts overhangs to blunt ends and ensures 5' phosphorylation for efficient adapter ligation.
  • A-tailing: Addition of a single adenine nucleotide to the 3' ends of blunt-ended fragments (for some platforms).
  • Adapter ligation: Attachment of platform-specific adapters to the DNA fragments, enabling amplification and sequencing.