Library amplification

DRAFT

This page is a work in progress and is subject to change at any moment.

Many sequencing protocols require amplification of the adapted DNA fragments:

• PCR amplification: Selectively amplifies fragments with correctly ligated adapters.
• Emulsion PCR: Used in some platforms to amplify DNA on beads in isolated microdroplets.
• Bridge amplification: An solid-phase amplification method used in some next-generation sequencing platforms.